Phospho-histone h2ax ser139
WebIn eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. WebThe AlphaLISA SureFire Ultra Phospho-Histone H2A.X (Ser139) assay kit is an immunoassay for quantitative detection of phosphorylated Histone H2A.X in cellular lysates. We are now two standalone organizations Life Sciences & Diagnostics and Analytical & Enterprise Solutions
Phospho-histone h2ax ser139
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WebH2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry. … WebPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at serine 139. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
WebHistone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand … WebThis unit describes immunocytochemical detection of histone H2AX phosphorylated on Ser-139 (γH2AX) to reveal DNA damage, particularly when the damage involves the presence of DNA double-strand breaks (DSBs). These breaks often result from DNA damage induced by ionizing radiation or by treatment with anticancer drugs such as DNA topoisomerase ...
WebAnti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho … WebFeb 4, 2010 · Abstract. Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming γH2AX, is an early cellular response to the induction of DNA double-strand breaks. Detection of this ...
WebThe H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4).
WebThe AlphaLISA SureFire Ultra Phospho-Histone H2A.X (Ser139) assay kit is an immunoassay for quantitative detection of phosphorylated Histone H2A.X in cellular … immigration attorneys seattle waWebAffordable TaqMan Assays for All of Your qPCR Needs immigration attorneys phoenix arizonaWebPhospho-H2A.X (Ser139) Antibody detects endogenous levels of H2A.X only when phosphorylated at Ser139. Species Reactivity: Human, Mouse, Rat, … list of systemically important nbfcWebLinear peptide corresponding to phospho-Histone H2A.X (Ser139). Application Anti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor ® 555 Conjugate is an antibody against phospho-H2A.X (Ser139) for use in Immunocytochemistry. list of synonyms of difficult wordsWebAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, … list of system and application softwareWebThe PathScan® Phospho-Histone H2A.X (Ser139) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H2A.X protein phosphorylated at Ser139. A histone H2A.X rabbit antibody has been coated onto the microwells. immigration attorney tipton countyWebSep 13, 2012 · By contrast, H2AX phosphorylation is not regulated by ATR or other H2AX-related kinases, such as JNK. Interestingly, ATM and DNA-PK phosphorylate H2AX at Ser139 in a synergistic manner with different kinetics of activation. Early after MNNG treatment, ATM generates γH2AX. Further, DNA-PK contributes to H2AX Ser139 phosphorylation. immigration attorney the woodlands