Differentiated hl60 pma

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August undefined, 2024

WebMar 14, 2024 · THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and … WebJan 15, 1994 · These data suggest that activation of protein kinase C cannot functionally upregulate these β 1 integrins on undifferentiated HL60 cells. The development of PMA-stimulated β 1-dependent adherence after granulocytic differentiation of HL60 cells suggests that the differentiated HL60 cell is a useful model for investigating functional …

Differentiation of HL‑60 cells in serum‑free hematopoietic cell media en…

WebNov 25, 2013 · Indeed, LRP5 OE HL60 cells, induced by PMA, showed less differentiation than control cells and the differentiation markers CD11b and CD44 were significantly reduced. The expression of the CD11b adhesion molecule is a maturation marker for HL60 cells in response to differentiation, as more than 90% of cells became CD11b‐positive … WebDec 1, 2003 · However, between 16- and 32-μM PMA, both HL60 cell lines responded almost equally as the superoxide release of dHL60 cells reached plateau values at 16-μM PMA (21 nmol/10 6 cells/h). Thus, the ndHL60 are less responsive than the dHL60 at low concentrations of PMA (EC50 for ndHL60 12.3 μM and for ndHL60, 4.3 μM) but not at … fiction female authors

Differentiation of HL-60 cells in serum-free hematopoietic …

WebDifferentiated human promyelocytic leukemia HL-60 cells (dHL-60) have been used to study the mechanisms of NETs formation, as neutrophils have a short lifespan that limits … WebOct 30, 2024 · Results. We constructed an effective model of ATRA-induced HL-60 differentiation which described signaling and gene expression events following the addition of ATRA (Fig. 1 ). HL-60 is a NCI-60 ... WebOct 17, 2024 · As expected , PMA-differentiated THP-1 cells showed increased production of the inflammatory cytokines IL-1β, IL-6, TNF-α and the ... In brief, HL60 and differentiated HL60 cells were incubated with 50 μg/mL PtNPs for 6 h. After the treatments, the cells were washed and incubated with Annexin V-FITC for 15 min in the dark at room temperature fiction fernanda

The influence of agents differentiating HL-60 cells toward granulocyte

Fig 1. Effect of hyperglycemia and PMA on ROS …

WebApr 20, 2000 · Our data provide evidence that PMA-induced activation of ERK in HL60 cells leads to growth arrest and terminal differentiation. The MEK inhibitor PD098059 blocks … http://www.plumbingpros.com/index.html gretchen whitmer kidnappersWebJan 3, 2014 · First, to estimate whether HL60 acts as a surrogate neutrophils in NET formation process, we treated both primary human neutrophils and neutrophil-like differentiated HL60 cells with PMA, and 1) the amount of extracellularly released histone H3 and 2) chromatin decondensation process were compared between both cells. fiction feminist books

"WebFeb 11, 2024 · Quantification of NETs using PicoGreen indicated that these cells release DNA when stimulated with PMA or CI; however, differentiated HL-60 cells in serum-free medium appeared to produce more ... Functional properties of HL60 cells matured with all-trans-retinoic acid and DMSO: Differences in response to interleukin-8 and fMLP. Leuk … " - Differentiated hl60 pma

Differentiated hl60 pma

The phorbol 12-myristate-13-acetate differentiation protocol is

WebHL60, the human promyelocytic leukemia cell line, consists of cells that resemble promyelocytes but are transformed into more mature myeloid forms (polymorphonuclear … WebbioRxiv.org - the preprint server for Biology

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WebHL-60 cells were incubated for 24 h at 37 °C with 20 n m PMA to induce differentiation to macrophage-like cells, with 1.2% (v/v) Me 2 SO or 100 … WebApr 21, 2000 · Our data provide evidence that PMA-induced activation of ERK in HL60 cells leads to growth arrest and terminal differentiation. The MEK inhibitor PD098059 blocks …

WebMar 13, 2024 · Superoxide anion production. 2 × 10 6 HL-60 cells were treated with 55% ethanol extract of E. purpurea at 28 µg/mL in HBSS alone, or with PMA (400 ng/mL) in HBSS alone, or a combination of both PMA at 400 ng/ml and the 55% ethanol extract of E. purpurea at 28 µg/mL for 30 minutes at 37°C. A medium control containing cytochrome c … WebOct 17, 2024 · Morphology of PMA-Differentiated THP-1 Cells in the Presence of PtNPs. ... HL60 and differentiated HL60) were incubated with 50 μg/mL PtNPs for the proper time, then washed twice with RPMI and fixed for 45 min in a fixative solution (2% Glutaraldehyde in complete culture medium). The samples were centrifuged and the pellet fixed again …

WebNov 12, 2024 · An HL60 cell line, derived from an AML patient, in a suspension culture proliferates mainly (>90%) as promyelocytes. It can be induced to differentiate into … WebFeb 10, 2015 · HL60 cell is a tumor cell line that was isolated from a single patient with acute promyelocytic leukemia and can be differentiated in vitro into a variety of blood …

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WebJul 1, 2002 · Incubation of both cell types with 10 nM PMA induced a marked, at least 11-fold, ... Roles for βII-protein kinase C and ROCK1 in positive and negative signaling for superoxide anion generation in differentiated HL60 cells. … fiction filipino moviesWebPMA induced expression of RPTPb mRNA and protein as determined by RNase protection assay and indirect immunofluorescence studies, respectively. Vitamin D 3 and interferon-g, which stimulate differentiation of HL-60 cells into monocyte-like cells, also induced VacA sensitivity and expression of RPTPb mRNA, whereas 1.2% Me 2 fiction fiction fictionWebDec 15, 1998 · In the variant HL60 cells differentiated with Bt 2 cAMP, PMA appears to place the complex in a primed state awaiting further stimulus before the NADPH oxidase response is elicited. The aforementioned PI3K and MEK-independent pathway provides the switch to turn on the oxidase complex. The extremely low concentrations of … gretchen whitmer kidnapping planWebJan 12, 2024 · HL-60 cells were differentiated with 1.3% v/v DMSO for 6 days with antibiotics or 5 μM of ATRA for 4 days without media change. For nuclei staining HL-60 neutrophil-like cells were treated with 0 ... gretchen whitmer kidnapping fbi plotWebPhagocytosis of fluorescent microspheres by HL-60 promyelocytic leukemia cells following induction of differentiation with dimethyl sulfoxide (DMSO) was monitored using flow … gretchen whitmer leather jacketWebPMA-induced NET formation occured sequentially in both neutrophil-like differentiated HL60 cells and primary neutrophils, and Rab27a-knockdown clearly inhibited NET … fiction fight 2WebThese results showed that differentiated HL60 cells produce NETs upon stimulation with PMA and E. coli [8, 9]. However, treatment with A. phagocytophilum did not significantly alter the MPO levels ... fiction filmmaking minor msu