WebOct 7, 2016 · HeLa cervix adenocarcinoma (ATCC CCL-2), K-562 chronic myelogenous leukemia (ATCC CCL-243), NCI-H460 lung cancer (ATCC HTB-177) and HGC27 gastric cancer (ATCC CRL-2506) cells were obtained from ... WebSep 1, 2010 · The combined treatment with TNF and CHX induces cell death through a caspase-dependent mechanism. Representative death indices for HeLa cells (−), HeLa cells treated with TNF, cycloheximide (CHX), TNF and CHX, TNF, CHX and DMSO, or TNF, CHX and the pan-caspase inhibitor z-VAD-fmk in a resazurin viability assay.
Chemotherapy-induced pyroptosis is mediated by …
WebMurder for Hire Misguided Mom. A teenage mother starts to make questionable choices when the father of her two children comes back into their lives; when the court grants … Web(E) HeLa cells were transfected with the destabilized nLuc-3XFlag-CL1/PEST reporters for 24 h and then collected (control) or treated for 3 h with 100 µg/mL CHX, 200 µM NSC119893, or 100 µg/mL CHX + 200 µM NSC119893. Luminescence signals for each reporter were quantified and set relative to the associated control samples. orange lady bugs that bite
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WebCell survival assays showed that HeLa cells of stably expressing MARVELD1-V5 (HeLa/MARVELD1) had reduced cellular sensitivity to hydroxyurea (HU), camptothecin (CPT) and aphidicolin (Aph) compared to pcDNA3.1-transfected control cells ... d The half-life of PARP1 was detected by WB in HeLa cells treated with CHX for the indicated time … WebJul 21, 2024 · 1 answer May 19, 2024 Greetings, Recently, I performed a Cycloheximide chase assay to assess the stability of transiently transfected proteins in HEK293 cells. The CHX chase assay was conducted... WebJan 13, 2003 · SCAT3-expressing HeLa cells were exposed to 10 μg/ml CHX or 50 ng/ml TNF-α/10 μg/ml CHX for 6 h, then washed three times in PBS at 37°C. The cells were resuspended in PBS and used for fluorescence spectra determinations with an excitation wavelength of 435 nm at 37°C using a fluorescence spectrophotometer (model F2500; … iphone stecker