Biotin synthase

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August undefined, 2024

WebMar 29, 1994 · This article is cited by 117 publications. Syam Sundar Neti, Debangsu Sil, Douglas M. Warui, Olga A. Esakova, Amy E. Solinski, Dante A. Serrano, Carsten Krebs, Squire J. Booker. Characterization of LipS1 … Webbiotin synthase also serves as the immediate sulfur donor for the dethiobiotin to biotin conversion (4, 12). The Fe-S cluster of biotin synthase may therefore have a dual function, and Fe-S cluster forming proteins, such as IscS, IscU and IscA, may thus be required for catalytic activity (13–15). However, the mech-

Microbial Cell Factories for Green Production of Vitamins

WebJun 17, 2024 · If the native biotin operon is overexpressed in B. subtilis, most enzymes will be strongly inhibited by the by-product of SAM. However, the high demand for SAM by biotin synthase and 7,8-diaminononanoate synthase is still a bottleneck that must be addressed in future research. WebAug 13, 2024 · Biotin is a water-soluble B-vitamin, also called vitamin B7 and formerly known as vitamin H or coenzyme R. It is composed of a ureido ring fused with a tetrahydrothiophene ring. ... Biotin synthase reductively cleaves SAM into a deoxyadenosyl radical—a first radical formed on dethiobiotin is trapped by the sulfur donor, which was … greek postal codes yellow pages

Biotin synthase DrugBank Online

WebBiotin synthase catalyzes formation of a thiophane ring through a radical mechanism that is difficult to reconstitute in vitro. Choi-Rhee and Cronan measure a turnover of 20-60 equivalents of biotin in vivo, but also find that turnover renders the protein susceptible to proteolytic destruction. WebNov 1, 2012 · Highlights The biotin biosynthetic pathway ends with insertion of a sulfur atom. Biotin synthase contains two iron-sulfur clusters and is an AdoMet radical … WebBiotin synthase belongs to the family of AdoMet-dependent enzymes that reductively cleave AdoMet into a deoxyadenosyl radical, and it is responsible for the homolytic cleavage of C-H bonds. A first radical formed on dethiobiotin is trapped by the sulfur donor, which was found to be the iron-sulfur (Fe-S) center contained in the enzyme, and ... greek population in montreal

The primary step of biotin synthesis in mycobacteria PNAS

Biotin Synthase - Chemistry & Biology

WebSep 30, 2003 · Biotin synthase catalyzes the radical-mediated insertion of sulfur into dethiobiotin to form biotin. The structure places the substrates between the Fe4S4 cluster, essential for radical generation, and the Fe2S2 cluster, postulated to be the source of sulfur, with both clusters in unprecedented coordination environments. ... WebBiotin synthase specifically converts dethiobiotin into biotin using this general reaction by introducing a sulfur atom between the C-6 and C-9 carbons of dethiobiotin (1). Biotin synthase begins by inserting the substrate between the Fe 4 S 4 iron cluster, the site of radical production, and the Fe 2 S 4 cluster, the primary source of sulfur. greek pork chops recipeWebFeb 28, 2006 · Biotin synthase, a member of the “radical SAM” family, catalyzes the final step of the biotin biosynthetic pathway, namely, the insertion of a sulfur atom into dethiobiotin. The as-isolated enzyme … flower curtain holdbacks

"WebApr 14, 2024 · Biotin is a heterocyclic, sulfur-containing monocarboxylic acid, and a coenzyme for carboxylase enzymes. It is also involved in gluconeogenesis, the synthesis of fatty acids, and the metabolism of fats and amino acids. Proteolysis by biotinidase is required prior to absorption because most biotin in foods is bound to protein. " - Biotin synthase

Biotin synthase

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WebBiotin synthase (BioB) catalyzes the insertion of a sulfur atom between the C6 and C9 carbons of dethiobiotin. Reconstituted BioB from Escherichia coli contains a [4Fe-4S]2+/1+ cluster thought to be involved in the reduction and cleavage of S-adenosylmethionine (AdoMet), generating methionine and the reactive 5′-deoxyadenosyl radical responsible … WebJan 11, 2024 · Biotin synthase folds into a closed triosephosphate isomerase type (α/β) 8 barrel flanked with two helices at the N-terminus and an unstructured C-terminal region. …

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WebAug 8, 2010 · Biotin synthesis is known to include a pimelate intermediate, the construction of which is controversial. ... Choi-Rhee, E. & Cronan, J.E. Biotin synthase is catalytic in … WebBiotin synthase (BioB) catalyzes the oxidative insertion of a sulfur atom between the C6 methylene and the C9 methyl positions in dethiobiotin. The enzyme couples oxidation of each carbon position to reduction of the S -adenosyl- l -methionine (SAM) sulfonium center, generating 5′-deoxyadenosine and l -methionine, products that are ...

WebThe [2Fe–2S] cluster has been proposed to be the sulfur donor in the biotin synthase reaction, which is consistent with previous suggestions that an iron–sulfur cluster was the … WebJun 9, 2016 · National Center for Biotechnology Information

WebMar 29, 1994 · Biotin Synthase Mechanism: Evidence for Hydrogen Transfer from the Substrate into Deoxyadenosine. Journal of the American Chemical Society 1999, 121 (15) , 3571-3578. … WebApr 7, 1999 · Biotin synthase, the enzyme which catalyses the conversion of dethiobiotin (DTB) to biotin has an absolute requirement for S-adenosylmethionine (AdoMet) which …

WebAug 13, 2024 · A key result presented here is that a large share of potential biotin auxotrophic bacteria encodes the gene for the biotin synthase (bioB) which catalyzes the final step of the biotin pathway, and ...

WebFeb 1, 1998 · Biotin-dependent enzymes contain a biotinyl-lysine residue in a conserved sequence motif, MKM, located in a surface hairpin turn in one of the two beta-sheets that make up the domain. A sub-gene encoding the 82-residue C-terminal biotinyl domain from the biotin carboxy carrier protein of acetyl-CoA … greek pork ribs recipeWebMar 3, 1998 · A cell-free extract from Escherichia coli containing an E. coli biotin synthase that was expressed to approx. 1% of soluble cell protein by cloning the E. coli bioB gene was used to investigate the biotin synthase reaction. The pH optimum was between ... greek postal service trackingWebAug 17, 2024 · Biotin is a covalently attached enzyme cofactor required for intermediary metabolism in all three domains of life. Several important human pathogens (e.g. … greek porticoWebApr 1, 2005 · Biotin synthase catalyzes formation of a thiophane ring through a radical mechanism that is difficult to reconstitute in vitro. Choi-Rhee and Cronan [1] measure a turnover of 20–60 equivalents of biotin in vivo, but also find that turnover renders the protein susceptible to proteolytic destruction. greek postal service reliabilityWebThe E. coli biotin synthase (BioB) catalyzes the insertion of sulfur into desthiobiotin to form biotin, which is then ligated to the biotin carboxy carrier protein (BCCP) by the biotin protein ... greek post trackingWebThe biotin synthesis pathway of E. coli and alignments of BioC and Tam proteins. (A) The E. coli BioC-BioH pathway for the synthesis of pimelate comprises three main steps.The … flower curtain diyWebbiotin synthase purified from E. coli cells grown on [35S]cys-teine produced [35S]biotin [34]. A possible hypothesis was that the (Fe-S) centre of the enzyme was the sulphur … greek posited geocentric theory